Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences

Table 3 Differentially expressed genes among three types of directly isolated cell

B fibroblasts/S fibroblasts		Myofibroblasts/S fibroblasts		Myofibroblasts/B fibroblasts
Gene	Fold change	Gene	Fold change	Gene	Fold change
Col1a1	2.97	Col1a1	3.91	P4ha2	2.68
Col1a2	2.05	Col1a2	2.28	Adamts2	0.42
Col3a1	3.02	P4ha2	2.68	Ccl5	0.32
P4ha3	11.76	P4ha3	21.01	Ccl7	0.19
Plod1	2.43	P4hb	2.49	Ccl11	0.02
Plod2	2.23	Plod1	2.30	Cxcl1	0.12
Lox	5.90	Plod2	2.88	Cxcl2	0.27
Loxl2	2.69	Plod3	2.06	Cxcl5	0.11
Ccl2	2.27	Lox	8.08	Cxcl10	0.49
Ccl7	2.10	Loxl2	5.27	Cxcl12	0.17
		Ccl7	0.40
		Ccl11	0.01
		Cxcl1	0.16
		Cxcl2	0.46
		Cxcl12	0.22
		Cxcl14	2.22

Differentially expressed genes in fibroblasts of bleomycin-injured lungs at day 12 (B fibroblasts) relative to fibroblasts of saline-treated lungs (S fibroblasts), myofibroblasts of bleomycin-injured lungs at day 12 relative to S fibroblasts, and myofibroblasts relative to B fibroblasts by a factor of > 2.0 or < 0.5 and P < 0.05. QRT-PCR was performed in triplicate using three independently prepared RNA samples. Results represent the mean of three experiments.

ISSN: 1755-1536