Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences

Table 2 Identification of cell surface markers distinguishing myofibroblasts and fibroblasts

Gene	ΔCt of lin^negof saline-treated lungs	ΔCt of lin^negof bleomycin-injured lungs of day 12	ΔΔCt	Fold change (Bleo/saline)
CD49e	9.7	6.8	−2.9	5.50^*
CD44	8.4	7.0	−1.4	2.28^*
CD29	7.9	7.1	−0.8	1.60
CD140b	8.8	8.8	0.0	1.00
CD147	8.8	8.9	0.1	0.94
CD120a	6.8	7.0	0.2	0.89
CD140a	7.5	8.0	0.5	0.75
CD107a	8.2	8.8	0.6	0.70
CD47	8.4	9.2	0.8	0.62^*
CD81	8.0	9.0	1.0	0.56^*
CD119	7.8	8.9	1.1	0.52^*
CD54	7.7	9.0	1.3	0.47^*
Sca-1	6.6	8.0	1.4	0.44^*

ΔCt = Ct of a gene of the sample minus Ct of 18 s rRNA of the sample. ΔΔCt = ΔCt of lin^neg of bleomycin-injured lungs at day 12 minus ΔCt of lin^neg of saline-treated lungs. Calculation for fold change in gene levels between bleomycin-injured lungs at day 12 and saline-treated lungs (bleomycin/saline) is described in Methods. QRT-PCR was performed in triplicate using three independently prepared RNA samples. Results represent the mean of three experiments. *, P < 0.05.

ISSN: 1755-1536