In-vitro and in-vivo proliferative capacity of myofibroblasts and fibroblasts. (A) Two hundred sorted cells from populations A, B, and C were grown in 12-well plates. The graph represents the number of adherent cells of each population, which were counted on days 1, 4, 7, and 10 after sorting. Experiments were performed in triplicate. Results represent the mean (± s.d.) of three experiments. *, P < 0.01. (B) The left figure shows the cell-cycle distribution of the DNA histogram from Hoechst 33342-stained cells of population A. The high peak on the left side represents cells in the G0G1 phase, and the low peak on the right side represents cells in the G2M phase. The region between the two peaks represents cells in the S phase. The proportion of cells in the G2M phase was compared among lineage-specific cell surface marker-positive cells of saline-treated lungs (lineage-positive cells of saline-treated lungs), and populations A, B, and C (right). The bar graph represents the proportion of cells in the G2M phase. Experiments were performed in triplicate. Results represent the mean (± s.d.) of three experiments. Asterisks indicate P < 0.001. s.d., standard deviation.