Figure 6

Identification of myofibroblast-rich and fibroblast-rich populations. (A) Flow cytometry plots showing the expression of CD49e (x-axis) and Sca-1 (y-axis) of lin^neg cells of saline-treated lungs (left) and bleomycin-injured lungs at day 12 (right). Lin^neg Sca-1^high of saline-treated lungs (population A) and bleomycin-injured lungs at day 12 (population B) are indicated as circles. Lin^neg Sca-1^low CD49e^high of bleomycin-injured lungs at day 12 (population C) and lin^neg Sca-1^low CD49e^low of bleomycin-injured lungs at day 12 (population D) are indicated as oblongs. Figures show representative results. (B) Histogram showing the expression levels of intracellular α-SMA of populations A, B, C, and D using FITC-conjugated anti-α-SMA antibody. As a control, FITC-conjugated isotype control antibody was used. Figures show representative results. (C) Sorted cells from populations A, B, C, and D were grown for 36 h, fixed in 4% paraformaldehyde, and subjected to immunocytochemistry using anti-α-SMA antibody (green), anti-vimentin antibody (red), and DAPI (blue). Phase-contrast images of living cells are shown in lower panels. Figures show representative results. Scale bars indicate 100 μm. DAPI, 4′,6-diamidino-2-phenylindole.