Figure 5

Exploring cell surface markers of myofibroblasts and fibroblasts. (A) Snap-frozen bleomycin-injured lungs at day 12 were fixed in acetone and examined by immunofluorescence using anti-α-SMA antibody (upper left), anti-CD49e antibody (upper right), and APC-conjugated anti-Sca-1 antibody (lower left); the three images were merged (lower right). Arrows indicate a myofibroblast. Figures show representative results. Scale bars indicate 100 μm. (B) Snap-frozen bleomycin-injured lungs at day 12 were fixed in acetone and examined by immunofluorescence with DAPI (upper left), FITC-conjugated antibodies to lineage-specific cell surface markers (CD31, CD45, EpCAM, TER119, CD146, and Lyve-1) (upper right), and PE-conjugated anti-Sca-1 antibody (lower left); the three images were merged (lower right). Arrows indicate cells that were localized in the adventitia of the pulmonary artery and that were negative for lineage-specific cell surface markers (upper right) but positive for Sca-1 (lower left). Figures show representative results. Scale bars indicate 50 μm. DAPI, 4′,6-diamidino-2-phenylindole.