Skip to main content
Figure 4 | Fibrogenesis & Tissue Repair

Figure 4

From: Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences

Figure 4

Myofibroblasts and fibroblasts were enriched in linnegcells of saline-treated lungs and bleomycin-injured lungs. (A) Expression levels of Acta2 and Col1a1 mRNA were compared among unfractionated cells of saline-treated lungs, linneg cells of saline-treated lungs, unfractionated cells of bleomycin-injured lungs at day 12, and linneg cells of bleomycin-injured lungs at day 12 using qRT-PCR. Results were normalized to expression levels of unfractionated cells of saline-treated lungs. QRT-PCR was performed in triplicate using three independently prepared RNA samples. Results represent the mean (± s.d.) of three experiments. *, P < 0.01. (B) Expression levels of intracellular α-SMA were compared between linneg cells of bleomycin-injured lungs at day 12 (left) and linneg cells of saline-treated lungs (right) using FITC-conjugated anti-α-SMA antibody. As shown in Figure 2C, 10,000 sorted linneg cells were fixed in 10% formalin and then permeabilized and incubated with mouse FITC-conjugated isotype control antibody (IgG2a) or FITC-conjugated anti-α-SMA antibody. The fluorescence intensity of FITC-α-SMA (black line) or FITC-conjugated isotype control (gray line) of linneg cells is shown. Figures show representative results. Unfrac, unfractionated; s.d., standard deviation.

Back to article page