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Figure 3 | Fibrogenesis & Tissue Repair

Figure 3

From: Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences

Figure 3

Lineage-specific cell surface markers were not expressed in myofibroblasts or perivascular cells. (A) Snap-frozen bleomycin-injured lungs at day 12 were fixed in acetone and examined by immunofluorescence using FITC-conjugated antibodies to lineage-specific cell surface markers (CD31, CD45, EpCAM, TER119, CD146, and Lyve-1) (left) and anti-α-SMA antibody (middle); these two images were merged with DAPI (blue in right). Figures show representative results. Scale bars indicate 100 μm. (B) Snap-frozen saline-treated lungs were fixed in acetone and examined by immunofluorescence with DAPI (left) and FITC-conjugated antibodies to lineage-specific cell surface markers (CD31, CD45, EpCAM, TER119, CD146, and Lyve-1) (middle); these two images were merged (right). Arrows indicate a cell that was localized in the adventitia of the pulmonary artery and that was negative for lineage-specific cell surface markers. The asterisk indicates the pulmonary artery. Figures show representative results. Scale bars indicate 50 μm. (C) Single lung cells, as shown in Additional file1: Figure S3 were plotted for APC-conjugated anti-CD31, CD45, EpCAM, and TER119 antibodies vs. FITC-conjugated anti-CD146 and Lyve-1 antibodies. Unfractionated cells were sorted from the square in the figure. Linneg cells were sorted from the APC and FITC double-negative fraction, as shown in the oblong. Unfrac, unfractionated; DAPI, 4′,6-diamidino-2-phenylindole.

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