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Figure 1 | Fibrogenesis & Tissue Repair

Figure 1

From: Direct isolation of myofibroblasts and fibroblasts from bleomycin-injured lungs reveals their functional similarities and differences

Figure 1

Characterization of the bleomycin-injured lung model. (A) Mice (solid line, N = 10) were intratracheally administered bleomycin. In mice treated with bleomycin, mortality was as high as 70% by day 12. (B) Saline-treated lungs at day 12 (N = 3) and bleomycin-injured lungs at day 12 (N = 3) were homogenized at the same time. Hydroxyproline content was higher in bleomycin-injured lungs at day 12 than in saline-treated lungs. Results represent the mean (± s.d.) of three experiments. (C) BAL fluid of saline-treated lungs (N = 3) was collected at day 12. BAL fluid of bleomycin-treated lungs was collected at days 3 (N = 3), 6 (N = 3), and 12 (N = 5). IL-6 and TGF-β1 concentration in BAL fluid at days 3, 6, and 12 was significantly higher than that of saline-treated lungs. Results represent the mean (± s.d.) of three or five experiments. (D) Total and differential cell count in BAL fluid of mice treated by bleomycin or saline (dashed line). BAL fluid of saline-treated lungs was collected at days 12 (N = 3). BAL fluid of bleomycin-treated lungs was collected at days 3 (N = 3), 6 (N = 3), and 12 (N = 5). Dashed line indicates the average of BAL fluid of saline-treated lungs. Results represent the mean (± s.d.) of three or five experiments. *, P < 0.05; **, P < 0.001; ***, P < 0.0001. BAL, bronchoalveolar lavage; s.d., standard deviation.

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