Table 4 Amplicons, genomic coordinates and primer sequences of the extended promoter regions measured
Amplicon | Genomic coordinates | Primer sequence | CpG | |
|---|---|---|---|---|
ACTA2 (1) | chr10:90,750,187-90,750,540 | Fwd | 5'-TAGTTAGGGTTGGTTTTAGGGTGT-3' | 19 |
Rev | 5'-CCTAAAATAAACATACCAACCACTACA-3' | |||
ACTA2 (2) | chr10:90,750,828-90,751,169 | Fwd | 5'-TTTGTTTTGAAGGTTGTAGGTTTTTT-3' | 26 |
Rev | 5'-ACTATTAAAACCTTCCCTCAAACCC-3' | |||
COL1A1 (1) | chr17:48,278,603-48,278,899 | Fwd | 5'-AGTTTATATGTTTAGGGTTTAGATATGTT-3' | 19 |
Rev | 5'-CCAAAATAAACTCCCTCCTATCTCA-3' | |||
COL1A1 (2) | chr17:48,278,860-48,279,232 | Fwd | 5'-AGTATTTTTGGTTTAGGTTGGG-3' | 17 |
Rev | 5'-CACAAAACTAAACATATCTAAACCCT-3' | |||