Table 3 Primer sequences of selected fibrogenic genes

GAPDH

Forward primer: 5'- GTCCACTGGCGTCTTCACCA -3'

Reverse primer: 5'- GTGGCAGTGATGGCATGGAC -3'

ACTA2

Forward primer: 5'- TTCAATGTCCCAGCCATGTA-3'

Reverse primer: 5'- GAAGGAATAGCCACGCTCAG-3'

FZD8

Forward primer: 5'- AGACAGGCCAGATCGCTAACT-3'

Reverse primer: 5'- AAGCGCTCCATGTCGATAAG-3'

NOX4

Forward primer: 5'- GGCCAGAGTATCACTACCTCC-3'

Reverse primer: 5'- GTTCGGCACATGGGTAAA-3'

TSPAN2

Forward primer: 5'- TTCATGTGTGATCTGCGTGTT-3'

Reverse primer: 5'- TGGGAGCGAAATAGGTTGT-3'