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Table 2 DNA gene methylation levels remain unchanged after TGF-β1 pulses

From: Cellular re- and de-programming by microenvironmental memory: why short TGF-β1 pulses can have long effects

Amplicon

CpG site probed

4 h TGF-β1 pulse

2 × 4 h TGF-β1 pulses

Day 1

Day 7

Day 14

Day 1

Day 7

Day 14

ACTA2 (1)

11

0.5 ± 0.5

0.85 ± 0.18

0.43 ± 0.33

0.86 ± 0.54

1.0 ± 0.41

1.75 ± 0.63

12

1.0 ± 0.47

1.33 ± 0.63

0.6 ± 0.39

1.5 ± 1.06

0.67 ± 0.33

0.83 ± 0.23

14

0.97 ± 0.18

1.26 ± 0.19

3.3 ± 2.26

1.58 ± 1.12

1.65 ± 0.36

0.7 ± 0.41

16

0.81 ± 0.43

0.78 ± 0.50

0.93 ± 0.54

0.98 ± 0.29

1.78 ± 0.53

1.0 ± 0.36

17

1.07 ± 0.32

1.08 ± 0.64

1.33 ± 0.63

1.2 ± 0.96

3.67 ± 2.92

0.5 ± 0.18

20.21

1.0 ± 0.52

1.0 ± 0.1

0.71 ± 0.51

1.0 ± 0.51

1.0 ± 0.81

1.0 ± 0.67

22

0.71 ± 0.25

1.4 ± 0.93

1.0 ± 0.71

0.43 ± 0.1

0.5 ± 0.33

1.0 ± 0.4

23

0.71 ± 0.83

2.0 ± 1.54

2.0 ± 1.41

1.25 ± 1.06

1.76 ± 1.5

2.0 ± 1.18

24

0.99 ± 0.63

1.2 ± 0.66

1.0 ± 0.29

0.71 ± 0.5

0.67 ± 0.31

2.0 ± 1.5

25

1.0 ± 0.67

1.5 ± 1.23

1.0 ± 0.51

1.0 ± 0.3

1.0 ± 0.67

1.0 ± 0.67

ACTA2 (2)

1

4.0 ± 2.65

2.33 ± 1.19

1.0 ± 0.67

0.83 ± 0.24

1.75 ± 0.6

0.67 ± 0.31

2

0.57 ± 0.11

1.67 ± 0.77

0.64 ± 0.15

1.14 ± 1.06

0.9 ± 0.78

0.56 ± 0.18

4

1.31 ± 0.36

0.94 ± 0.08

0.57 ± 0.27

0.97 ± 0.41

1.15 ± 0.17

0.84 ± 0.25

5

1.0 ± 0.67

2.0 ± 1.41

0.6 ± 0.32

0.33 ± 0.47

0.8 ± 0.23

1.0 ± 0.89

10

0.99 ± −.67

0.83 ± 0.23

0.75 ± 0.35

1.75 ± 0.63

1.0 ± 0.47

1.0 ± 0.4

11

2.0 ± 1.82

2.5 ± 0.71

0.33 ± 0.49

0.5 ± 0.39

1.0 ± 0.71

1.67 ± 0.92

12.13

1.75 ± 0.36

0.4 ± 0.12

0.83 ± 0.46

1.0 ± 0.89

0.5 ± 0.18

1.0 ± 0.29

14.15.16

0.69 ± 0.13

0.9 ± 0.14

0.81 ± 0.17

0.73 ± 0.45

0.78 ± 0.38

0.73 ± 0.35

COL1A1 (1)

3

1.0 ± 0.40

1.5 ± 0.5

1.33 ± 0.63

1.54 ± 0.43

1.0 ± 0.45

1.40 ± 0.39

8.19

0.67 ± 0.56

0.60 ± 0.33

0.75 ± 0.53

0.92 ± 0.24

0.91 ± 0.28

0.73 ± 0.12

COL1A1 (2)

2

1.0 ± 0.81

1.33 ± 0.3

1.0 ± 0.47

1.67 ± 0.7

1.0 ± 0.71

0.83 ± 0.45

7

1.4 ± 0.49

0.5 ± 0.33

1.75 ± 0.35

1.0 ± 0.71

1.5 ± 0.35

1.62 ± 0.60

8.9

0.92 ± 0.59

2.0 ± 0.58

0.36 ± 0.41

1.33 ± 0.98

0.57 ± 0.38

1.4 ± 0.94

 

11.12

0.67 ± 0.32

2.0 ± 1.8

1.0 ± 0.67

0.75 ± 0.35

0.75 ± 0.45

0.7 ± 0.51

  1. ACTA2 and COL1A1 were not regulated by DNA methylation in response to TGF-β1 pulse(s). Changes in methylation levels >10% are considered significant. ACTA2 and COL1A1 genes expressed low methylation levels and no overt changes after TGF-β1 pulse(s). ACTA1 actin alpha 1, COL1A1 collagen, type I, alpha 1, TGF-β1 transforming growth factor-β1.