Skip to main content

Table 1 Selected fibrosis-related genes were upregulated post-TGF-β1 pulse

From: Cellular re- and de-programming by microenvironmental memory: why short TGF-β1 pulses can have long effects

Model

Single pulse - 0.5 h, 4 h

Double pulse - 2 × 0.5 h, 2 × 4 h

Gene/day

1

7

14

1

7

14

α-SMA

20 ± 2.6*

1.3 ± 0.4

0.8 ± 0.2

20 ± 2.9*

5.6 ± 1.3*

1.2 ± 0.2

FZD8

90 ± 25*

2 ± 0.8*

1.1 ± 0.2

100 ± 30*

25 ± 11*

2.3 ± 1.5

NOX4

50 ± 25*

1.4 ± 0.4

0.9 ± 0.1

74 ± 29*

9.3 ± 2.5*

1.6 ± 0.4

TSPAN2

500 ± 100*

3 ± 1.5*

1.5 ± 0.3

240 ± 70*

20 ± 7.8*

2.9 ± 1.1*

  1. Fibroblasts were pulsed with or without TGF-β1 at the indicated time points. The expression of selected genes was quantified by RT-PCR analysis. Data are represented as averages of fold changes of TGF-β1 pulse-induced gene expression ± SD, quantified from triplicate studies in duplicate, and expressed as fold changes over respective controls (rounded to 1 dp). *P <0.05 versus respective untreated controls. α-SMA α-smooth muscle actin, FZD8 frizzled family receptor 8, NOX4 NADPH oxidase 4, TSPAN2 transmembrane glycoprotein tetraspanin 2.