Figure 1
A TGF-β1 pulse(s) elevated the collagen I secretion rate. (a) Growth-arrested fibroblasts were treated with or without TGF-β1 according to the cell culture setup comparing single and double TGF-β1 pulses with the traditional 4 days of TGF-β1 treatment. Normalised densitometric SDS-PAGE analysis of the 24 h collagen secretion rate of pulsed fibroblasts for the (b) 0.5 h, (c) 4 h; (d) 2 × 0.5 h, (e) 2 × 4 h TGF-β1 pulses, and (f) 4 days of TGF-β1 treatment. *P <0.05 versus respective untreated controls. Data are represented as mean ± SD, calculated from three independent studies in triplicate, and expressed as fold changes over respective controls. SD, standard deviation; TGF-β1 transforming growth factor-β1.