Figure 6
The effect on migration of dermal and lung fibroblasts after treatment with PDGFRα and PDGFRβ neutralising antibodies. Dermal fibroblasts were grown in 10% serum. A scratch was made in the cell layer and cells were treated with mitomycin C with neutralising antibodies to either anti-PDGFRα, anti-PDGFRβ, anti-PDGFRα and anti-PDGFRβ, control IgG, Imatinib or 10% FCS for 1 h at room temperature and then stimulated with PDGF-AA, PDGF-BB, PDGF-CC or PDGF-DD. (a). Percentage migration was calculated by measuring the average density of cells migrated into the scratched area after 24 h (b-f). Error bars are standard errors of the mean. Two-sample T-test was performed - 10% FCS vs. Media + mitomycin C, 10% FCS vs. IgG treated, anti-PDGFRα + anti-PDGFRβ vs. anti-PDGFRα + anti-PDGFRβ + PDGF-BB, Imatinib vs. Imatinib + PDGF-BB, p = 0.032, 0.035, 0.92, and 0.1 respectively. Anti-PDGFRα + PDGF-AA vs. PDGF-AA, anti-PDGFRβ + PDGF-AA vs. PDGF-AA, anti-PDGFRβ + PDGF-BB vs. PDGF-BB, anti-PDGFRα + PDGF-BB vs. PDGF-BB, anti-PDGFRβ + PDGF-CC vs. PDGF-CC, anti-PDGFRα + PDGF-CC vs. PDGF-CC, anti-PDGFRβ + PDGF-DD vs. PDGF-DD, anti-PDGFRα + PDGF-DD vs. PDGF-DD —p = 0.24, 0.93, 0.006, 0.24, 0.06, 0.34, 0.38 and 0.64 respectively. Lung fibroblasts were treated and analysed in identical fashion. (g-k). Two-sample T-test was performed - FCS vs. Media + mitomycin C, 10% FCS vs. IgG treated, anti-PDGFRα + anti-PDGFRβ vs. anti-PDGFRα + anti-PDGFRβ + PDGF-BB, Imatinib vs. Imatinib + PDGF-BB —p = 0.585, 0.18, 0.64 and 0.21 respectively. Anti-PDGFRα + PDGF-AA vs. PDGF-AA, anti-PDGFRβ + PDGF-AA vs. PDGF-AA, anti-PDGFRβ + PDGF-BB vs. PDGF-BB, anti-PDGFRα + PDGF-BB vs. PDGF-BB, anti-PDGFRβ + PDGF-CC vs. PDGF-CC, anti-PDGFRα + PDGF-CC vs. PDGF-CC, anti-PDGFRβ + PDGF-DD vs. PDGF-DD, anti-PDGFRα + PDGF-DD vs. PDGF-DD —p = 0.25, 0.75, 0.05, 0.64, 0.82, 0.69, 0.1 and 0.23 respectively.