Figure 5

The effect of collagen gel contraction of dermal fibroblasts after treatment with PDGFRα and PDGFRβ neutralising antibodies. Dermal fibroblasts were treated with neutralising antibodies to anti-PDGFRα, anti-PDGFRβ or blank for 1 h at room temperature and were added to a collagen gel. The gels were then grown in media containing either PDGF-AA or PDGF-BB. After 24 h the gels were weighed to assess contraction. Error bars are standard error of the mean. Two-sample T-test statistical analysis was performed compared to compared gel weights compared to the 0% FCS control p = 0.004 PDGF-AA, p = 0.032 PDGF-BB, p = 0.82 anti-PDGFR-α., p = 0.62 anti-PDGFR-α.+ PDGF-AA, p = 0.03 anti-PDGFR-α.+ PDGF-BB, p = 0.77 anti-PDGFR-β, p = 0.035 anti-PDGFR-β + PDGF-AA, p = 0.0007 anti-PDGFR-β + PDGF-BB. *p > 0.05.