Figure 3

Phosphorylation of PDGFRα and PDGFRβ in response different doses of PDGF-AA and PDGF-CC ligands. Dermal fibroblasts were grown in 10% FCS and serum-starved overnight. Cells were stimulated with 0% FCS, PDGF-BB, PDGF-AA (5-200ng/ml) and PDGF-CC (5-200ng/ml) for 15 min. Whole cell lysates were Western blotted using antibodies against phospho-PDGFRα, phospho-PDGFRβ and GAPDH (loading control).