Figure 2From: Platelet-derived growth factor alpha and beta receptors have overlapping functional activities towards fibroblastsPhosphorylation of PDGFRα and PDGFRβ in response to PDGF ligands. Dermal fibroblasts (a) and lung fibroblasts (b) were grown in 10% FCS and serum-starved overnight or kept in 10% FCS. Cells were then stimulated with either 10% FCS, 0% FCS, PDGF-AA, PDGF-BB, PDGF-DD or Imatinib for 15 min. Whole cell lysate were Western blotted using antibodies against phospho-PDGFRα, PDGFRα, phospho-PDGFRβ, PDGFRβ and GAPDH (loading control).Back to article page