Figure 1

A. Effect of Pravastatin on mRNA expression of TGFβ1, CTGF and Collagen in a kinetic manner: Twenty four hours kinetics of mRNA expression of TGFβ1, CTGF, and Col Iα2 in control and Pravastatin treated cells show that Pravastatin treatment with a dose of 0.1 mM and up reduces levels of mRNAs of these genes with maximum efficiency at six hours post-treatment; C: control, P: Pravastatin treatment. 0.1, 0.5, and 1 refer to treatment dose in mM. *: p < 0.05, **: p < 0.01, ***: p < 0.005 according to kruskal-Wallis test. B. Protein expression of CTGF in control and Pravastatin treated cells: Pravastatin treatment for 24 hours inhibits CTGF protein expression showing also a dose-response relationship. GAPDH is used as a housekeeping gene. C: control, P: Pravastatin treatment. 0.1, 0.5 refer to treatment dose in mM.