Figure 4

Alpha-SMA expression is enhanced in PPARγ KO (K/K) wound tissue (day 7 and day 10 post-wounding). (A) Indirect immunofluorescence analysis of WT (C/C) and PPARγ KO (K/K) mice with an anti-α-SMA antibody (C/C: N = 10; K/K: N = 12, original magnification × 20, bar = 50 μm). Green, α-SMA; Blue, DAPI. (B) Quantification of α-SMA expression intensity in wound tissues at day 7 and day 10 after wounding. (C) Western blot of α -SMA expression in WT (C/C) and PPARγ KO (K/K) wound tissues at day 7 after wounding. GAPDH = loading control. (D) Indirect immunofluorescence analysis of WT (C/C) and PPARγ KO (K/K) mice with an anti-neutrophil antibody (C/C: N = 10; K/K: N = 12, original magnification × 20, bar = 100 μm). Red, neutrophils; Blue, DAPI. (E) Quantification of neutrophils in wound tissues at day 7 after wounding. Asterisks indicate a significant difference between C/C and K/K groups (** = P < 0.01; *** = P < 0.001). DAPI, 4',6-diamidino-2-phenylindole; PPARγ, peroxisome proliferator-activated receptor-γ.