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Figure 4 | Fibrogenesis & Tissue Repair

Figure 4

From: Discoidin domain receptors regulate the migration of primary human lung fibroblasts through collagen matrices

Figure 4

Normal human lung fibroblast (NHLF) migration through collagen IV is matrix metalloproteinase (MMP)-2-dependent. NHLFs were reverse transfected with negative control small interfering (si)RNA, or specific siRNA for MMP-10, or MMP-2 using lipofectamine RNAiMAX. At 48 h after transfection, NHLFs were serum-starved for 24 h and transferred to 8.0 μm polycarbonate inserts coated collagen IV (10 μg/cm2). Cells were incubated with collagen I (25 μg/mL) or vehicle (acetic acid, 0.1 M) for an additional 16 h. The bottom chamber was treated with cell dissociation buffer and acetomethoxycalcein (calcein AM) for 1 h at 37°C. The fluorescence of solution with detached cells was measured at 485 nm excitation and 520 nm emission. Results are representative of mean fold increase ± SD of two independent experiments performed in triplicate (n = 6, *P < 0.05).

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