Table 1 A 30-plex gene expression panel, including five human gene normalization controls and the two human ribosomal (DNA and RNA) controls used in singleplex
Gene | Gene name | GenBank accession | Rationale |
|---|---|---|---|
18S ribosomal DNA | . | NR_003286 | Tissue Quality & Quantity Assessment |
28S ribosomal RNA | . | NR_003287 | “ “ |
ACTB | Actin, beta | NM_001101 | Normalization |
PPIA | Peptidylprolyl isomerase A (cyclophilin A) | NM_021130 | “ “ |
RPL13A | Ribosomal protein L13a | NM_012423 | “ “ |
TBP | TATA box binding protein | NM_003194 | “ “ |
UBC | Ubiquitin C | NM_021009 | “ “ |
ACTA2 | Actin, alpha 2, smooth muscle, aorta | NM_001613 | Fibrosis and/or HSC Activation Marker |
ANXA2 | Annexin A2 | NM_001002857 | “ “ |
ARNT2 | Aryl-hydrocarbon receptor nuclear translocator 2 | NM_014862 | HSC Housekeeping |
COL1A1 | Collagen, type I, alpha 1 | NM_000088 | Fibrosis and/or HSC Activation Marker |
COL3A1 | Collagen, type III, alpha 1 | NM_000090 | “ “ |
COL4A1 | Collagen, type IV, alpha 1 | NM_001845 | “ “ |
CPEB1 | Cytoplasmic polyadenylation element binding protein 1 | NM_030594 | HSC Housekeeping |
CTGF | Connective tissue growth factor | NM_001901 | Fibrosis and/or HSC Activation Marker |
CTHRC1 | Collagen triple helix repeat containing 1 | NM_138455 | “ “ |
CXCL2 | Chemokine (C-X-C motif) ligand 2 ; CINC-2a, GROb, Gro2, MIP-2, MIP-2a | NM_002089 | “ “ |
EDN1 | Endothelin 1 | NM_001955 | “ “ |
FOXF1 | Forkhead box F1 | NM_001451 | HSC Housekeeping |
HAMP | Hepcidin antimicrobial peptide | NM_021175 | Fibrosis and/or HSC Activation Marker |
HSPA1A | Heat shock 70 kDa protein 1A | NM_005345 | “ “ |
IGFBP6 | Insulin-like growth factor binding protein 6 | NM_002178 | HSC Housekeeping |
KRT18 | Keratin 18; cytokeratin 18, cell proliferation inducing protein 46 | NM_000224 | Fibrosis and/or HSC Activation Marker |
LOXL2 | Lysyl oxidase-like 2 | NM_002318 | “ “ |
MRGPRF | MAS-related GPR, member F | NM_145015 | HSC Housekeeping |
P311 | Neuronal protein 3.1, C5orf13 | NM_004772 | “ “ |
SYP | Synaptophysin | NM_003179 | Fibrosis and/or HSC Activation Marker |
TGFB1 | Transforming growth factor, beta 1 | NM_000660 | “ “ |
TGFB2 | Transforming growth factor, beta 2 | NM_003238 | “ “ |
TIMP1 | TIMP metallopeptidase inhibitor 1 | NM_003254 | “ “ |
TNF | Tumor necrosis factor (TNF superfamily, member 2) | NM_000594 | “ “ |
TUBB | Tubulin, beta | NM_178014 | HSC Housekeeping & Fibrosis Marker |