Skip to main content
Figure 6 | Fibrogenesis & Tissue Repair

Figure 6

From: Thrombospondin 1 is a key mediator of transforming growth factor β-mediated cell contractility in systemic sclerosis via a mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK)-dependent mechanism

Figure 6

The overexpression of thrombospondin 1 (TSP1) in systemic sclerosis (SSc) fibroblasts is dependent on endogenous transforming growth factor (TGF)β and platelet-derived growth factor (PDGF) via mitogen-activated protein kinase kinase (MEK)/extracellular signal-regulated kinase (ERK) activation. SSc fibroblasts were pretreated with SB431542 (activin-linked kinase 5 (ALK5) inhibitor), U0126 (ERK inhibitor), interferon (IFN)β or Gleevec (PDGF receptor inhibitor) overnight, prior to determining TSP1 gene and protein expression. (a) Western blotting showing that SB431542, U0126, Gleevec and IFNβ inhibited the overexpression of TSP1 protein in SSc fibroblasts. (b) The mRNAs of fibroblasts in these treated samples were also assayed by reverse transcription (RT)-PCR.

Back to article page

Fibrogenesis & Tissue Repair

ISSN: 1755-1536

Contact us