Figure 3
From: Ha-Ras stabilization mediates pro-fibrotic signals in dermal fibroblasts
pSmad3 and collagen type I kinetics in Ha-Ras-infected mouse dermal fibroblast (mDF). (A) pSmad3 and Smad3 and (B) collagen I immunoblots (n = 3 per each sample) of protein extracts prepared at the indicated time points after infection of mDF cultures with Ha-Ras or control lentiviruses with bar graphs on the side summarizing the ratios of pSmad3 or collagen I relative to the loading control glyceraldehyde 3-phosphate dehydrogenase (GADPH) in the various experimental samples; asterisks indicate statistically significant differences (P < 0.05). (C) Real-time quantitative polymerase chain reaction (qPCR) estimates of Smad3 transcript levels in mDF cultures infected with Ha-Ras or control lentiviruses. In the first panel, the arrow and arrowhead respectively point to pSmad3 and an unidentified cross-reacting receptor-activated Smad (R-Smad).