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Table 3 Identification of Integra® (IT) matrix targeting peptides from peptide libraries after bovine serum albumin (BSA), phosphate-buffered saline (PBS) and non-fat milk (NFM) blockinga

From: A phage-targeting strategy for the design of spatiotemporal drug delivery from grafted matrices

IT8b R3 (pH 2) IT8 R3 (NaCl) IT8 R3 (pH 2) IT8 R3 (NaCl) IT8 R4 (pH 2) IT8 R4 (pH 2) IT9c R4 (pH 2)
BSA IT8-BA BSA IT8-BAS PO4 IT8-PA PO4 IT8-PAS- BSA IT8-BA PO4 IT8-PA- NFM IT9-MA
Figure 6A Figure 6B Figure 6C Figure 6D Figure 7
BA1 CVPSSARIC BAS1 CHMSPRHQS PA1 CVQSSTQHC PAS4 CHVTAQRAC R4BA1 CKGPVSRHC R4PA1 CGKHDDTYC MA1 HETFPSPRANSV
BA2 CRPHDSKAC BAS2 CLPNKQWSC PA2 CSGHHSLRC PAS5 CATPEWPPC R4BA2 CTTSSEHVC R4PA2 CPTKDLRYC MA2 HETFPSPRANSV
BA3 CHPEPRSQC BAS3 CKQPLNNTC PA3 CPTSQQKVC PAS6 CPNLMNTRC R4BA3 CSTTMKTSC R4PA3 CTSSGNRYC MA3 HETFPSPRANSV
BA4 CVEKRPRQC BAS4 CTVTPRHLC PA4 CNSTHPRAC PAS8 CTKSSPPRC R4BA4 CDNKRSPAC R4PA4 CTIKTNLQC MA4 HETFPSPRANSV
BA5 CFMDYRNLC BAS5 CDNTSKTQC PA5 CNRLESHLC PAS9 CTQTTVASC R4BA5 CKLNYPNAC R4PA5 CHSTAKSAC MA5 SQIDYATGPRQA
BA6 CSHSVQPFC BAS6 CASTTAACC PA6 CTNPHRSQC PAS10 CDQSKTIAC R4BA7 CQFSKSQSC R4PA6 CPASKGDFC MA6 WDTEKASPLSPL
BA7 CQTHNPRQC BAS7 CLHMDKKRC PA7 CTKTPWPGC PAS11 CSRGSMGIC R4BA8 CTLDTRRDC R4PA8 CSHRVPHDC MA7 HETFPSPRANSV
BA8 CDGAPAPLC BAS8 CMKTPMRSC PA8 CNRLQGEHC PAS12 CSPIRGSMC R4BA10 CPFSSSPSC R4AP9 CHATPYPKC MA8 HETFPSPRANSV
BA10 CKTDLQKQC BAS10 CYKHVGQRC PA9 CPTPTGRYC PAS13 CSHTGHHQC R4BA11 CPSMSHHQC R4PA10 CDSSRHTHC MA10 DHTGKSPGLFHN
BA11 CGPFPQPHC BAS11 CHLSPFKSC PA10 CVPTAMSNC PAS14 CHEPTTMAC R4BA12 CSASTQSFC R4PA11 CSRLSQEYC - -
BA12 CSFHGPGPC BAS12 CTTSKYRDC PA11 CSLARPNEC PAS15 CSRADLTTC R4BA13 CPLKGLATC R4PA12 CTGKQYPQC - -
BA13 CSTNQTPTC BAS13 CTATGLSNC PA12 CVRTPFSMC - - R4BA14 CTGKPLKTC R4PA13 CGMNAFRAC A1 AHKHKHPGHITA
BA14 CSTSPONSC BAS14 CPSSMPSRC PA13 CNNTTPPSC - - R4BA17 CIHMTGYHC R4PA14 CGMNAFRAC - -
BA15 CKLIHNNSC BAS15 CPATSHTHC PA15 CTSQQKANC - - R4BA21 CEMTETKHC R4PA16 CTTKYSTTC - -
- - - - - - - - R4BA26 CKENWPLIC R4PA18 CSSDKALVC - -
- - - - - - - - R4BA30 CSNSPTTMC R4PA24 CSPRSHLSC - -
- - - - - - - - - - R4PA25 CPPSPMPYC - -
  1. aThe sequences of peptides pulled from the biopanning strategies IT8 and IT9 are presented along with their code numbers for reference.
  2. bIT8 was screened against matrix sonicated samples blocked with BSA or PBS-T, and the peptide sequences were generated from both acid and GAG elutions.
  3. cIT9 was biopanned from thin sections of matrix blocked with NFM or PBS-T, and only the acid/high salt eluates evaluated.