A phage-targeting strategy for the design of spatiotemporal drug delivery from grafted matrices

Table 1 Summarized biopanning strategies used^a for the identification of Integra^® (IT) matrix targeting peptides from New England Biolabs libraries

Protocol	IT1BA	IT6BA	IT6BAS	IT6BGC	IT7BA	IT7BCG	IT8BA	IT8BAS	IT8PA	IT8PAS	IT9MA	IT9PA
Target	Matrix	Sonicated	Sonicated	Sonicated	Sonicated	Sonicated	Sections	Sections	Sections	Sections	Sonicated	Sonicated
Library	C7C	C7C	C7C	C7C	PHD12	PHD12	C7C	C7C	C7C	C7C	PHD12	PHD12
Block	BSA	BSA	BSA	BSA	BSA	BSA	BSA	BSA	PO₄	PO₄	NFM	PO₄
Elution	Acid	Acid	Acid/salt	GAG	Acid	GAG	Acid	Acid/salt	Acid	Acid/salt	Acid	Acid
Selection rounds	3 to 5	3	3	3	3	3	4	3	4	3	3	3
Colonies analyzed	50	15	15	15	15	15	30	30	30	30	30	30
Peptide ID	Table 2	Table 2	Table 2	Table 2	Table 2	Table 2	Table 3	Table 3	Table 3	Table 3	Table 3	Table 3
Phylogeny	Figure 3	Figure 4A	Figure 4A	Figure 5A	Figure 4B	Figure 5B	Figure 6A,C	Figure 6A	Figure 6B,D	Figure 6B	Figure 7	Figure 7

^aFour distinct strategies were evaluated (IT1, IT7, IT8 and IT9), which involved changing the blocking buffer (phosphate-buffered saline with Tween, bovine serum albumin (BSA) and non-fat milk (NFM)), the elution conditions (acid, acid/salt and glaycosaminoglycan), the target (matrix, sonicated samples and thin sections) and the number of rounds (three or four) of biopanning.

ISSN: 1755-1536