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Table 1 Summarized biopanning strategies useda for the identification of Integra® (IT) matrix targeting peptides from New England Biolabs libraries

From: A phage-targeting strategy for the design of spatiotemporal drug delivery from grafted matrices

Protocol IT1BA IT6BA IT6BAS IT6BGC IT7BA IT7BCG IT8BA IT8BAS IT8PA IT8PAS IT9MA IT9PA
Target Matrix Sonicated Sonicated Sonicated Sonicated Sonicated Sections Sections Sections Sections Sonicated Sonicated
Library C7C C7C C7C C7C PHD12 PHD12 C7C C7C C7C C7C PHD12 PHD12
Block BSA BSA BSA BSA BSA BSA BSA BSA PO4 PO4 NFM PO4
Elution Acid Acid Acid/salt GAG Acid GAG Acid Acid/salt Acid Acid/salt Acid Acid
Selection rounds 3 to 5 3 3 3 3 3 4 3 4 3 3 3
Colonies analyzed 50 15 15 15 15 15 30 30 30 30 30 30
Peptide ID Table 2 Table 2 Table 2 Table 2 Table 2 Table 2 Table 3 Table 3 Table 3 Table 3 Table 3 Table 3
Phylogeny Figure 3 Figure 4A Figure 4A Figure 5A Figure 4B Figure 5B Figure 6A,C Figure 6A Figure 6B,D Figure 6B Figure 7 Figure 7
  1. aFour distinct strategies were evaluated (IT1, IT7, IT8 and IT9), which involved changing the blocking buffer (phosphate-buffered saline with Tween, bovine serum albumin (BSA) and non-fat milk (NFM)), the elution conditions (acid, acid/salt and glaycosaminoglycan), the target (matrix, sonicated samples and thin sections) and the number of rounds (three or four) of biopanning.