Phylogenic tree of peptide targeting candidates using chondroitin sulfate elution from matrix sonicated samples. Peptide sequences were obtained after the third round of screening using (A) the IT6 protocol, which biopanned using the C7C library or (B) the IT7 protocol, which used the PHD12 library as starting material. In both instances the phage were recovered from the chondroitin sulfate (CS)-glycosaminoglycan (GAG) eluates of washed sonicated matrix samples, which contained CG to remove bound peptides. Sequences were analyzed for structural relatedness using MacVector sequence analysis software. Library collapse was indicated by an increased recovery of 400-fold in IT7 over the three rounds of biopanning.