Impaired dermal wound healing in discoidin domain receptor 2-deficient mice associated with defective extracellular matrix remodeling

Table 1 mRNA Levels of extracellular matrix (ECM) molecules in cultured discoidin domain receptor 2 (DDR2)^+/+ and DDR2^-/^- skin fibroblasts

mRNA	DDR2^+/+	DDR2^-/-	Expression when DDR2 is absent
Col I (α 1)	2.54 ± 0.04	1.34 ± 0.11	Down
Col VI (α 2)	0.31 ± 0.07	0.45 ± 0.05	Up
Col VIII (α 1)	0.56 ± 0.02	0.34 ± 0.05	Down
Syndecan1	1.01 ± 0.06	0.06 ± 0.10	Down
Fibrillin 1	0.95 ± 0.01	0.78 ± 0.02	Down
Fibronectin	0.17 ± 0.01	0.13 ± 0.04	NS
α2	0.92 ± 0.03	1.52 ± 0.03	Up
α3	0.67 ± 0.07	0.61 ± 0.04	NS
DDR1	0.94 ± 0.20	0.91 ± 0.38	NS
MMP2	0.94 ± 0.01	0.31 ± 0.08	Down
MMP9	ND	ND	ND
MMP10	0.57 ± 0.09	0.24 ± 0.03	Down
MMP13	1.34 ± 0.10	1.17 ± 0.09	NS

Relative mRNA expression of ECM molecules regulated by DDR2 expression in DDR2^+/+ and DDR2^-/- skin fibroblasts. Gene expression was normalized to RPL13 mRNA expression. ND indicates mRNA was not detected. Results are expressed as means ± standard error of the mean (SEM) (n = 3). All P < 0.05 except for NS.
MMP = matrix metalloproteinase; NS = not significantly different.

ISSN: 1755-1536