Figure 3

Hyaluronidase (HYAL) induces mononuclear cell increase in bronchoalveolar space by mechanism partially dependent on lipid mediators. (A) Leukotriene (LT)B₄ presents in the supernatant of lung homogenates were analysed by specific electroimmunoassay. C57Bl/6 was inoculated intranasally (i.n.) with 16 U of bovine testicular HYAL or phosphate buffered saline (PBS) and the lungs were removed 96 h later. Values represent means ± standard error of mean (SEM); n = 10; P = 0.04 compared to the PBS; t test was used. (B) Prostaglandin E₂ concentration in the supernatant of lung homogenates was analysed by specific electroimmunoassay. C57Bl/6 was inoculated i.n. with 16 U of bovine testicular HYAL or PBS and the lung was removed 96 h later. Values represent means ± SEM; n = 10; P = 0.15 compared to the PBS group, t test was used. (C) Pre-treatment with dexamethasone (1 mg/kg s.c.); MK886 (5 mg/kg/0.5 mL by gavage) or celecoxib (5 mg/kg/0.5 mL by gavage) significantly reduced the number of cells in bronchoalveolar space of C57Bl/6 mice. All treatments were performed 1 h before stimuli and every 24 h until the end of experiment. Control received only PBS. Values represent means ± SEM.; n = 5; * P > 0.01 compared to PBS; # P > 0.001 compared to HYAL + PBS, ANOVA test was used. (C) Mononuclear cells recruitment to the bronchoalveolar space of 5-LO ^-/- (129-Alox^5tm1Fun) mice is decrease when compared to strain-matched wild-type (WT) mice. Each strain was inoculated with 16 U of HYAL and cells recovered 96 h after stimulus. Values represent means ± SEM; n = 5; * P < 0.0001 compared to PBS WT; ^# P = 0.0003 compared to PBS 5-LO ^-/-; ^# P = 0.0003 compared to HYAL WT; test t was used.