Figure 3
Positive chemokine (C-C motif) receptor 2 (CCR2) expression in human and murine blood fibrocytes by flow cytometry. Representative flow cytometric analysis of fibrocytes stained after primary passage with collagen I, CD45 and CCR2 or isotype controls. (C) Collagen I and CD45 staining of human fibrocytes with gate parameters calculated based in isotype controls (A and B). (D) Representative histogram of CCR2 expression (blue) in human fibrocyte population compared to IgY isotype control (red). (G) Collagen I and CD45 staining of murine fibrocytes with gate parameters calculated based in isotype controls (E and F). (H) Representative histogram of CCR2 expression (blue) and collagen I (green) compared to relevant isotype control (red). (I) Quantitative analysis of percentage of CCR2+ cells in fibrocyte population. Data are expressed as the mean ± SEM of n = 4 human donors and n = 30 mice.