Assessment of proteolytic degradation of the basement membrane: a fragment of type IV collagen as a biochemical marker for liver fibrosis

Table 4 Spiking recovery in human serum^a

Peptide concentration, ng/mL	Peptide serum + peptide concentration, ng/mL	Peptide recovered, ng/mL	Recovery, %
31.1	145.3	31.1	103.5
13.4	135.3	13.3	110.4
7.6	125.7	7.6	107.6
4.7	121.6	4.7	106.7
0	109.2	0	100

^aPeptide sample = native type IV collagen cleaved by matrix metalloproteinase 9 and pepsin. Recovery percentages are peptide concentrations compared to peptide measured in serum after subtracting the serum background.

ISSN: 1755-1536