Figure 2
Immunoprecipitation of CO3-610 type III collagen fragment from rat serum. CO3-610 was precipitated with monoclonal antibodies from serum of a cirrhotic (F) rat treated with CCl4 for 19 weeks and an untreated age-matched control (C) rat and detected by western blotting as a single discrete protein band of 25 kDa. The numbers on the right are the molecular masses of Full-Range Rainbow Markers (Mr 12,000 to 225,000) from GE Healthcare (Little Chalfont, United Kingdom). The figure is representative of three independent experiments.