Table 1 Primer and Probe sequences for real-time RT-PCR
Gene | Oligo | Sequence 5'→3' |
|---|---|---|
Glycoprotein Ba | Forward | CGCTCATTACGGCCCAAA |
Reverse | ACCACGCCCTGGACAACTC | |
Probe | TTGCCTATGACAAGCTGACCACCA | |
β-actin | Forward | CCGTGAAAAGATGACCCAGATC |
Reverse | CACAGCCTGGATGGCTACGT | |
Probe | TTTGAGACCTTCAACACCCCCAGCCA | |
Interferon-β | Forward | TGACGGAGAAGATGCAGAAGAG |
Reverse | TGGAGTTCATCCAGGAGACGTA | |
Probe | TGCCTTTGCCATCCAAGAGATGCTC | |
Toll-like receptor-9 | Forward | GAGTACTTGATGTGGGTGGGAATT |
Reverse | GCCACATTCTATACAGGGATTGG | |
Probe | CCGTCGCTGCGACCATGCC | |
DNA polymerase | Forward | ACAGCAGCTGGCCATAAAGG |
Reverse | TCCTGCCCTGGAAAGTGATG | |
Probe | CCTCTGGAATGTTGCCTTGCCTCCA |