Figure 6

TLR-9^-/- mice are defective in interferon (IFN)-β production. (A) Balb/c or TLR-9^-/- mice were injected with bleomycin on day 0. On day 14 mice received γHV68 intranasally. Lungs were harvested on days 0, 14, 17 and 21, and lung homogenates were prepared and analyzed by ELISA for IFN-β (n = 5 mice/group). (B) Fibroblasts isolated from Balb/c or TLR-9^-/- mice were infected in vitro with 0.01 PFU γHV68 and total RNA was prepared after 24 h of infection for analysis of IFN-β by real-time RT-PCR. The mean of the Balb/c samples was set to 1, n = 5/group.