Figure 5
Inflammatory responses are similar in Balb/c and TLR-9-/- mice. Mice were treated with bleomycin on day 0, and were given 5 × 104 PFU γHV68 intranasally on day 14. On day 21, lungs were collected and subjected to digestion with collagenase and DNAse. (A) Lung leukocytes were enumerated (n = 4 mice/group, representative of four experiments). (B) Isolated lung leukocytes were examined by flow cytometry for the percentage surface expression of CD4, CD8, CD19, Gr-1 (PMNs) and DX5 (NK cells). Samples were gated on CD45+ cells first. Percentages of lymphocytes were then determined within a lymphocyte gate. Gr-1 percentages were gated on CD45+ non-lymphocyte sized cells (n = 4/group representative of two experiments). **P < 0.01 for CD8 in TLR-9-/- mice treated with bleomycin plus γHV68 compared with Balb/c with same treatment. For B cells, virally infected mice were significantly different from mice treated with bleomycin alone in both genotypes (P < 0.01).