Phenotype of SSc-ILD and normal monocytes. PBMCs were isolated and analyzed by using flow cytometry as indicated for their expression of ColI, CD11b, CD14 and CD34. Each symbol represents the results obtained with cells from a different donor. The median value obtained in each category for SSc-ILD patients and normal donors is indicated by a line. (A) Double-labeled analysis. Note that among CD11b+ monocytes, CD14+ cells are more prevalent among SSc-ILD cells than among normal cells. (B) Triple- and quadruple-labeled analyses. Note that CD14+/ColI+, CD14+/CD34+/ColI+, and CD14-/CD34+/ColI+ cells are more prevalent among SSc-ILD CD11b+ monocytes than among normal CD11b+ monocytes. *P < 0.05.