Figure 4

Monocyte migration in vitro. The migration of normal and SSc-ILD monocytes toward CXCL12, the effect of TGFβ on normal monocyte migration and the ability of caveolin scaffolding domain (CSD) peptide to inhibit migration were quantified as described in Materials and methods. Each symbol represents the results obtained with cells from a different donor. The median value obtained in each category is indicated by a line. (A) Normal versus SSC-ILD ± CSD. Each symbol represents one subject. Note the almost sevenfold increase in migration in SSc-ILD lung tissue and the almost complete inhibition of migration by CSD peptide. (B) Normal ± transforming growth factor β (TGFβ) ± CSD. Each symbol represents one subject. Note the 15-fold increase in migration induced by TGFβ and the almost complete inhibition of migration by CSD peptide.