Figure 2

Stromal cell-derived factor 1 in human lung tissue. Tissue sections of normal and SSc-ILD lungs were stained with anti-stromal cell-derived factor 1 (anti-CXCL12) (red) and the nuclear stain DAPI (blue). (A) Normal lung tissue section at × 10 magnification. (B) SSc-ILD lung tissue section at × 10 magnification. (C) SSc-ILD lung tissue section at × 20 magnification. (D) SSc-ILD lung tissue section at × 20 magnification without primary antibody control. Note the massive CXCL12 staining in SSc-ILD lung tissue (B) and (C) and little staining in normal lung tissue (A). e, epithelial cells; M, macrophage. Bars = 10 μm.