Figure 1From: Elevated transforming growth factor β and mitogen-activated protein kinase pathways mediate fibrotic traits of Dupuytren's disease fibroblastsCharacterisation of Dupuytren's disease and control tissue specimens. (A) Immunostaining of Dupuytren's disease (DD) and control tissue specimens for transforming growth factor β3 (TGF-β3), phosphorylated Smad2 (P-Smad2), α-smooth muscle actin (α-SMA) and H & E. Representative staining from DD patient 4 and control patient 1 is shown. Insets within each image are higher-magnification views. Evaluation of all four control and DD tissue samples are described in Table 2. (B) Protein expression analysis for P-Smad2; phosphorylated Smad3 (P-Smad3); total Smad2, Smad3 and Smad1; α-SMA; type I collagen; and fibronectin/ED-A by Western blot analysis of 100 μg of total protein extract isolated from four individual control patient-derived and four individual DD patient-derived tissue samples. β-actin was used as a loading control.Back to article page