Induction of VEGF and TSP-1 in the media of HMEC-1 cells cultured under hypoxic conditions. Conditioned media from confluent HMEC-1 grown under normoxic or hypoxic culture conditions were assayed by ELISA for VEGF and TSP-1. Hypoxic culture (24 hours) was associated with increased levels of both (a) VEGF (mean ± SEM VEGF in normoxic conditions 184 ± 54 pg/mL, mean ± SEM VEGF in hypoxic conditions 447 ± 22 pg/mL; P < 0.05) and (b) TSP-1 (mean ± SEM TSP-1 in normoxic conditions 569 ± 37 pg/mL, mean ± SEM TSP-1 in hypoxic conditions 775 ± 24.5 pg/mL; P < 0.003). (c) In addition, TSP-1 mRNA levels were assayed by performing qRT-PCR. Hypoxic culture led to increased TSP-1 mRNA levels (mean ± SEM TSP-1 copy number in normoxia 3.19 ± 0.41, mean ± SEM TSP-1 copy number in hypoxia 8.98 ± 2.39; P < 0.004).