Figure 1

Flow cytometric detection of collagen containing monocytes in the transforming growth factor (TGF)-β1-exposed murine lung. (a,b) Fluorescein isothiocyanate (FITC)-detected intracellular isotype control (x axis) versus CD45-peridinin chlorophyll protein complex (PerCP) (y axis) in (a) Tg^- and (b) Tg⁺ mice. This control was used to set the negative gate for FITC in each animal respectively. A shift in autofluorescence is seen in the Tg⁺ animals. (c,d) FITC-detected collagen (Col)-Iα1 (x axis) versus CD45-PerCP (y axis) in (c) Tg- and (d) Tg⁺ mice. An increase in double positive cells is seen in the Tg⁺ animals. (e) Phycoerythrin (PE) -isotype control (x axis) versus CD45-anti-allophycocyanin (APC) (y axis). This control was used to set the negative gate for PE. (f) CD45-PE (x axis) versus APC-isotype control (y axis). This control was used to set the negative gate for APC. (g,h) CD34 (x axis) and CD14 (y axis) on the CD45⁺Col-Iα1⁺ population identified in (g) Tg^- and (h) Tg⁺ mice. In contrast to Tg- mice, in which the CD45⁺Col-Iα1⁺ cells express CD14 and CD34 to varying degrees, the CD45⁺Col-Iα1⁺ cells that appear in response to TGF-β1 overexpression are almost uniformly CD14⁺, with minimal contribution of CD34⁺ cells. N = 5 mice/group, images are representative of all animals.