Figure 6

Immunohistochemistry for CC chemokine receptor type 1 (CCR1), CCR3 and CCR5 on lung biopsy tissues. Representative immunohistochemistry for CCR1 (a-d), CCR3 (e-g), CCR5 (i-k, m), mast cells via tryptase (n) and appropriate isotype control antibodies (d, h, l) on lung biopsy tissue from stage II pulmonary sarcoidosis without alveolitis and on empiric therapy. CCR1 and CCR3 protein expression was found in epithelioid histiocytes and multinucleated giant cells forming non-necrotizing granulomas, as well as from surrounding alveolar macrophages and other infiltrating mononuclear cells. CCR5 protein expression was found in mast cells (i-k, m-n). The red arrows indicate mast cells (n) are expressing CCR5 (m). Panels were photographed at 50 × (a, d, e, h, i, l), 100 × (b, f, j, m, n) and 200 × (c, g, k) original magnification.