|  | Binding to CI |
---|
 |  | Without (GPO)10 | 10× (GPO)10 |
---|
 |  | Off rate (s-1) | K
d
(μM) | Off rate (s-1) | K
d
(μM) |
---|
MMP-2 | Pro | 0.48 | 0.07 ± 0.03 | 0.37 | 0.10 ± 0.04 |
 | Act | 0.48 | 0.17 ± 0.05 | 0.56 | 0.21 ± 0.15 |
MMP-9 | Pro | 0.32 | 0.12 ± 0.09 | 0.22 | 1.22 ± 0.64 |
 | Act | 0.76 | 0.87 ± 0.39 | 0.61 | 1.79 ± 1.81 |
- aMatrix metalloproteinases (MMPs) (100 nM) were passed over collagen type I (CI) immobilized to a sensor chip in the presence or absence of a 10-fold molar excess of Gly-Pro-Hyp triplets (GPO)10. MMP self-activation and activity were prevented by a buffer system consisting of phosphate-buffered saline and 0.05% (vol/vol) Tween 20. K
d
values and off rates of MMP binding to CI were determined by surface plasmon resonance (SPR) analysis and are shown as mean values ± SD from at least three experiments.