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Figure 4 | Fibrogenesis & Tissue Repair

Figure 4

From: Hydroxyproline-containing collagen analogs trigger the release and activation of collagen-sequestered proMMP-2 by competition with prodomain-derived peptide P33-42

Figure 4

Activation and activity of pro/actMMP-2 in the presence of (GPO) 10 and immobilized CI. (A) Activation of proMMP-2 (50 ng) or (B) activity of fully activated actMMP-2 alone or mixed with 10-fold molar excesses of (GPO)10 was determined in CI- or bovine serum albumin (BSA)-coated wells with 800 nM MCA-Pro-Leu-Gly-Leu-Dnp-Dap-Ala-Arg-NH2 substrate. Conversion of the fluorogenic substrate was monitored over time (data collection rate min-1) at 37°C. Graphs represent mean values of three independent experiments.

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