Activation and activity of pro/actMMP-2 in the presence of (GPO)
and immobilized CI. (A) Activation of proMMP-2 (50 ng) or (B) activity of fully activated actMMP-2 alone or mixed with 10-fold molar excesses of (GPO)10 was determined in CI- or bovine serum albumin (BSA)-coated wells with 800 nM MCA-Pro-Leu-Gly-Leu-Dnp-Dap-Ala-Arg-NH2 substrate. Conversion of the fluorogenic substrate was monitored over time (data collection rate min-1) at 37°C. Graphs represent mean values of three independent experiments.