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Figure 3 | Fibrogenesis & Tissue Repair

Figure 3

From: Hydroxyproline-containing collagen analogs trigger the release and activation of collagen-sequestered proMMP-2 by competition with prodomain-derived peptide P33-42

Figure 3

Binding of pro/actMMP-2/-9 to CI in the presence or absence of 10-fold molar excesses of Gly-Pro-Hyp triplets (GPO) 10 and MMP inhibitor. (A) ProMMP-9 and (B) proMMP-2 (25-150 nM) with or without a 10-fold molar excess of (GPO)10 in MMP activity buffer were left for 15 min on ice before being subjected to surface plasmon resonance (SPR) analysis with CI immobilized to a sensor chip. Association kinetics were changed to dissociation kinetics after 30 s. Pro(C)/act(D)MMP-2 (25-150 nM) with or without a 10-fold molar excess of (GPO)10 in MMP activity buffer containing 1 μM Ro 28-2653 were subjected to SPR analysis as described for (A and B). Association kinetics were changed to dissociation kinetics after 60 s. Plots after background subtraction from one experiment and one MMP concentration are shown from three independent experiments.

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Fibrogenesis & Tissue Repair

ISSN: 1755-1536

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