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Table 1 Real-time PCR values for indicated target genes in mRNA purified from tendon tissue samples.

From: Interleukins 4 and 13 modulate gene expression and promote proliferation of primary human tenocytes

Patient Tendon tissue State Ct, mean ± SDa
GAPDH IL-4R α IL-13R α2 IL-6
1 FDL Healthy 23.3 ± 0.3 25.8 ± 0.6 26.1 ± 0.1 32.1 ± 0.1
  PTT Diseased 23.8 ± 0.2 24.7 ± 0.1 24.4 ± 0.4 32.2 ± 0.1
2 FDL Healthy 27.5 ± 0.7 32.4 ± 0.5 33.2 ± 0.5 37.0 ± 0.6
  PTT Diseased 24.7 ± 0.5 26.7 ± 0.1 25.6 ± 0.6 37.7 ± 0.9
3 FDL Healthy 25.0 ± 0.4 30.0 ± 0.8 31.3 ± 0.7 34.1 ± 0.9
  PTT Diseased 26.1 ± 0.5 29.6 ± 0.6 21.8 ± 0.8 35.0 ± 0.8
4 FHL Healthy 26.5 ± 0.3 27.1 ± 0.2 ND 38.8 ± 0.8
  AT Diseased 27.3 ± 0.1 30.0 ± 0.3 ND U
5 FDL Healthy 27.9 ± 0.6 30.2 ± 0.5 31.1 ± 0.5 36.6 ± 0.7
  PTT Diseased 28.4 ± 0.7 30.0 ± 0.6 31.7 ± 0.7 35.8 ± 0.6
6 FDL Healthy 29.2 ± 0.9 32.4 ± 0.8 31.3 ± 0.7 U
  PTT Diseased 30.1 ± 0.9 32.2 ± 0.8 32.1 ± 0.9 U
7 FDL Healthy 29.1 ± 0.7 ND 28.8 ± 0.6 36.8 ± 0.9
  PTT Diseased 28.6 ± 0.6 ND 26.3 ± 0.5 37.0 ± 0.9
8 AT Healthy 25.5 ± 0.2 27.4 ± 0.4 27.5 ± 0.3 35.4 ± 0.8
   Diseased 20.2 ± 0.1 22.7 ± 0.3 24.5 ± 0.3 34.4 ± 0.7
9 AT Healthy 26.5 ± 0.4 29.3 ± 0.5 U 36.2 ± 0.7
   Diseased 26.5 ± 0.3 25.9 ± 0.3 25.9 ± 0.6 31.0 ± 0.6
  1. aEach cDNA sample was tested in triplicate.
  2. AT = Achilles tendon; FDL = flexor digitorum longus; FHL = flexor hallucis longus; ND = no data (not tested); PTT = posterior tibial tendon; U = undetermined (no amplification up to PCR cycle 40).
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