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Table 1 Real-time PCR values for indicated target genes in mRNA purified from tendon tissue samples.

From: Interleukins 4 and 13 modulate gene expression and promote proliferation of primary human tenocytes

Patient

Tendon tissue

State

Ct, mean ± SDa

GAPDH

IL-4R α

IL-13R α2

IL-6

1

FDL

Healthy

23.3 ± 0.3

25.8 ± 0.6

26.1 ± 0.1

32.1 ± 0.1

 

PTT

Diseased

23.8 ± 0.2

24.7 ± 0.1

24.4 ± 0.4

32.2 ± 0.1

2

FDL

Healthy

27.5 ± 0.7

32.4 ± 0.5

33.2 ± 0.5

37.0 ± 0.6

 

PTT

Diseased

24.7 ± 0.5

26.7 ± 0.1

25.6 ± 0.6

37.7 ± 0.9

3

FDL

Healthy

25.0 ± 0.4

30.0 ± 0.8

31.3 ± 0.7

34.1 ± 0.9

 

PTT

Diseased

26.1 ± 0.5

29.6 ± 0.6

21.8 ± 0.8

35.0 ± 0.8

4

FHL

Healthy

26.5 ± 0.3

27.1 ± 0.2

ND

38.8 ± 0.8

 

AT

Diseased

27.3 ± 0.1

30.0 ± 0.3

ND

U

5

FDL

Healthy

27.9 ± 0.6

30.2 ± 0.5

31.1 ± 0.5

36.6 ± 0.7

 

PTT

Diseased

28.4 ± 0.7

30.0 ± 0.6

31.7 ± 0.7

35.8 ± 0.6

6

FDL

Healthy

29.2 ± 0.9

32.4 ± 0.8

31.3 ± 0.7

U

 

PTT

Diseased

30.1 ± 0.9

32.2 ± 0.8

32.1 ± 0.9

U

7

FDL

Healthy

29.1 ± 0.7

ND

28.8 ± 0.6

36.8 ± 0.9

 

PTT

Diseased

28.6 ± 0.6

ND

26.3 ± 0.5

37.0 ± 0.9

8

AT

Healthy

25.5 ± 0.2

27.4 ± 0.4

27.5 ± 0.3

35.4 ± 0.8

  

Diseased

20.2 ± 0.1

22.7 ± 0.3

24.5 ± 0.3

34.4 ± 0.7

9

AT

Healthy

26.5 ± 0.4

29.3 ± 0.5

U

36.2 ± 0.7

  

Diseased

26.5 ± 0.3

25.9 ± 0.3

25.9 ± 0.6

31.0 ± 0.6

  1. aEach cDNA sample was tested in triplicate.
  2. AT = Achilles tendon; FDL = flexor digitorum longus; FHL = flexor hallucis longus; ND = no data (not tested); PTT = posterior tibial tendon; U = undetermined (no amplification up to PCR cycle 40).