Skip to main content
Figure 4 | Fibrogenesis & Tissue Repair

Figure 4

From: A novel soft-tissue in vitro model for bisphosphonate-associated osteonecrosis

Figure 4

Annexin V. (a) Annexin V apoptosis assay was performed in human gingival fibroblast (HGF) and HaCaT cells. First DDs treated with either normal medium in infusion solution (NM) or ZA 0.5, 1, 3, 5 or 10 μM and either left unchelated or exposed to EDTA 0.001% (chelated) were assayed. Secondly, the medium from the non-chelated (NC) or chelated (C) DDs was removed and applied to fresh HGF or HaCaT cells for 24 hours. (b) Cell proliferation results using mitochondrial tetrazolium salt (MTS) assay. First, DDs treated with either normal medium in infusion solution (NM) or ZA 0.5, 1, 3, 5 or 10 μM and either left unchelated or exposed to EDTA or EGTA 0.001% (chelated) were assayed. Secondly, the medium from the non-chelated (NC) or chelated (C-EDTA or C-EGTA) DDs was removed and applied to fresh HaCaT cells for 24 hours. Error bars indicate SEM. The growth of cells in NM was set as the control. *Significant (P < 0.05) differences from control cells.

Back to article page