Figure 2

Vital stain. Confocal microscopy of (a) human gingival fibroblast (HGF) and (b) HaCaT cells exposed to DDs treated with either normal medium (NM) with infusion solution or ZA 1 or 5 μM (shown as a lack of fluorescence at the right of the image) and either left unchelated (top rows) or exposed to EDTA 0.001% (chelated) (bottom rows). Images show cells that are viable (green), cells initiating cell death (yellow) and cells that are dying (red) Cells were observed over 24 hours with images taken at 24 hours (original magnification ×400).