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Figure 2 | Fibrogenesis & Tissue Repair

Figure 2

From: Autocrine transforming growth factor β signaling regulates extracellular signal-regulated kinase 1/2 phosphorylation via modulation of protein phosphatase 2A expression in scleroderma fibroblasts

Figure 2

Protein phosphatase 2A (PP2A) blockade stimulates extracellular signal-regulated kinase (ERK)1/2 phosphorylation in dermal fibroblasts. (a) Dermal fibroblasts were treated with 2 nm of okadaic acid (OA) for 1 h and protein levels of phosphorylated ERK1/2 were measured by western blot. (b) Normal dermal fibroblasts were treated with 100 nm PP2A siRNA or scramble control for 48 h. Cell extracts were then analyzed for phosphorylated ERK1/2 after PP2A blockade. (c) Bar graph shows quantification of phospho-ERK1/2 after treatment with PP2A siRNA; P < 0.05. β Actin was used as the loading control.

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