Figure 1

Transforming growth factor (TGF)β regulates extracellular signal-regulated kinase (ERK)1/2 phosphorylation and protein phosphatase 2A (PP2A) expression in dermal fibroblasts. (a) Adult dermal fibroblasts were treated with TGFβ (5 ng/ml) for 0 h, 15 min, 1 h, 3 h, 6 h, 12 h and 24 h after serum starvation. Western blot analysis was performed using phospho-ERK1/2 and ERK1/2 primary antibodies and rabbit horseradish peroxidase-conjugated secondary antibody. (b) Adult dermal fibroblasts were serum starved overnight and treated with 5 ng/ml of TGFβ for 0 h, 6 h, 12 h, 24 h and 48 h. RNA was then extracted and cDNA synthesized for quantitative PCR analysis; n = 3, *P < 0.05, **P < 0.01. (c) Dermal fibroblasts were treated for 24 h with 5 ng/ml of TGFβ after 24 h of serum starvation, and western blot analysis was performed using a PP2A antibody directed against the catalytic subunit; n = 5, representative blot of three experiments. (d) Bar graphs represent quantification of western blot analysis from (c); n = 5, *P < 0.05 versus normal control.