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Table 2 Conditioned media from LTA-treated PBMC, LPS-treated PBMC, and control conditioned media contain low levels of IL-6, TNF-α, IFN-γ, G-CSF and TGF-β1

From: Toll-like receptor 2 agonists inhibit human fibrocyte differentiation

Cytokine

Concentration in control CM (pg/ml)

Concentration in LPS-treated CM (pg/ml)

Concentration in LTA-treated CM (pg/ml)

IL-6

4 ± 12

530 ± 250

750 ± 250

TNF-α

4.6 ± 1.6

29 ± 13

33 ± 6

IFN-γ

1.0 ± 0.5

2.4 ± 0.9

4.5 ± 3.5

G-CSF

2.5 ± 0

17 ± 0

9.3 ± 1.8

TGF-β1

13 ± 1.8

3.5 ± 0

4.5 ± 0

  1. The above five cytokines were detected in conditioned media (CM) from LTA-treated PBMCs, LPS-treated PBMCs and control PBMCs. This assay measures total tumor growth factor (TGF)-β1 as opposed to active TGF-β1. The results are means ± SEM of cytokine concentration (n = 2 separate experiments).