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Table 2 Conditioned media from LTA-treated PBMC, LPS-treated PBMC, and control conditioned media contain low levels of IL-6, TNF-α, IFN-γ, G-CSF and TGF-β1

From: Toll-like receptor 2 agonists inhibit human fibrocyte differentiation

Cytokine Concentration in control CM (pg/ml) Concentration in LPS-treated CM (pg/ml) Concentration in LTA-treated CM (pg/ml)
IL-6 4 ± 12 530 ± 250 750 ± 250
TNF-α 4.6 ± 1.6 29 ± 13 33 ± 6
IFN-γ 1.0 ± 0.5 2.4 ± 0.9 4.5 ± 3.5
G-CSF 2.5 ± 0 17 ± 0 9.3 ± 1.8
TGF-β1 13 ± 1.8 3.5 ± 0 4.5 ± 0
  1. The above five cytokines were detected in conditioned media (CM) from LTA-treated PBMCs, LPS-treated PBMCs and control PBMCs. This assay measures total tumor growth factor (TGF)-β1 as opposed to active TGF-β1. The results are means ± SEM of cytokine concentration (n = 2 separate experiments).